Title :

Redox-labelled electrochemical aptasensors with nanosupported cancer cells

Author(s) :

Li, S. [Auteur]
The University of Tokyo [UTokyo]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Coffinier, Yannick [Auteur]
NanoBioInterfaces - IEMN [NBI - IEMN]
Lagadec, C. [Auteur]
Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] [UNICANCER/Lille]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Cleri, Fabrizio [Auteur]
Physique - IEMN [PHYSIQUE - IEMN]
Nishiguchi, K. [Auteur]
NTT Corporation
Fujiwara, A. [Auteur]
NTT Corporation
Fujii, T. [Auteur]
The University of Tokyo [UTokyo]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Kim, S.-H. [Auteur]
The University of Tokyo [UTokyo]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Clement, Nicolas [Auteur correspondant]
The University of Tokyo [UTokyo]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]

Journal title :

Biosensors and Bioelectronics

Pages :

114643, 7 pages

Publisher :

Elsevier

Publication date :

2022

ISSN :

0956-5663

English keyword(s) :

Bioelectrochemistry
Aptasensors
Nanopillars
Brownian motion
Nanoconfinement
Cancer cell

HAL domain(s) :

Sciences de l'ingénieur [physics]/Micro et nanotechnologies/Microélectronique
Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Biotechnologies
Sciences de l'ingénieur [physics]

English abstract : [en]

The transfer of redox-labelled bioelectrochemical sensors from proteins to cells is not straightforward because of the cell downward force issue on the surface of the sensors. In this paper, 20-nm-thick nanopillars are ...
Show more >The transfer of redox-labelled bioelectrochemical sensors from proteins to cells is not straightforward because of the cell downward force issue on the surface of the sensors. In this paper, 20-nm-thick nanopillars are introduced to overcome this issue, in a well-controlled manner. We show on both molecular dynamics simulations and experiments that suspending cells a few nanometers above an electrode surface enables redox-labelled tethered DNA aptamer probes to move freely, while remaining at an interaction distance from a target membrane protein, i. e. epithelial cell adhesion molecule (EpCAM), which is typically overexpressed in cancer cells. By this nanopillar configuration, the interaction of aptamer with cancer cells is clearly observable, with 13 cells as the lower limit of detection. Nanoconfinement induced by the gap between the electrode surface and the cell membrane appears to improve the limit of detection and to lower the melting temperature of DNA aptamer hairpins, offering an additional degree of freedom to optimize molecular recognition mechanisms. This novel nanosupported electrochemical DNA cell sensor scheme including Brownian-fluctuating redox species opens new opportunities for the design of all-electrical sensors using redox-labelled probes.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

ANR Project :

Comptage d'événements de transfert d'électron individuels dans les processus électrochimiques: une nouvelle approche en biodétection

Collections :

• Cancer Heterogeneity, Plasticity and Resistance to Therapies (CANTHER) - UMR 9020 - UMR 1277

Source :

Harvested from HAL