Titre :

Effects of Immunoglobulins G From Systemic Sclerosis Patients in Normal Dermal Fibroblasts: A Multi-Omics Study

Auteur(s) :

Chepy, Aurélien [Auteur]
Vivier, Solange [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Bray, Fabrice [Auteur]
Miniaturisation pour la Synthèse, l'Analyse et la Protéomique (MSAP) - USR 3290
Ternynck, Camille [Auteur]
Meneboo, Jean-Pascal [Auteur]
Figeac, Martin [Auteur]
Filiot, Alexandre [Auteur]
Guilbert, Lucile [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Jendoubi, Manel [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Rolando, Christian [Auteur]
Launay, David [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Dubucquoi, Sylvain [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Marot, Guillemette [Auteur]
MOdel for Data Analysis and Learning [MODAL]
METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694
Sobanski, Vincent [Auteur]

Titre de la revue :

Frontiers in Immunology

Nom court de la revue :

Front. Immunol.

Éditeur :

Frontiers Media SA

Date de publication :

2022-06-29

ISSN :

1664-3224

Résumé en anglais : [en]

Autoantibodies (Aabs) are frequent in systemic sclerosis (SSc). Although recognized as potent biomarkers, their pathogenic role is debated. This study explored the effect of purified immunoglobulin G (IgG) from SSc patients ...
Lire la suite >Autoantibodies (Aabs) are frequent in systemic sclerosis (SSc). Although recognized as potent biomarkers, their pathogenic role is debated. This study explored the effect of purified immunoglobulin G (IgG) from SSc patients on protein and mRNA expression of dermal fibroblasts (FBs) using an innovative multi-omics approach. Dermal FBs were cultured in the presence of sera or purified IgG from patients with diffuse cutaneous SSc (dcSSc), limited cutaneous SSc or healthy controls (HCs). The FB proteome and transcriptome were explored using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and microarray assays, respectively. Proteomic analysis identified 3,310 proteins. SSc sera and purified IgG induced singular protein profile patterns. These FB proteome changes depended on the Aab serotype, with a singular effect observed with purified IgG from anti-topoisomerase-I autoantibody (ATA) positive patients compared to HC or other SSc serotypes. IgG from ATA positive SSc patients induced enrichment in proteins involved in focal adhesion, cadherin binding, cytosolic part, or lytic vacuole. Multi-omics analysis was performed in two ways: first by restricting the analysis of the transcriptomic data to differentially expressed proteins; and secondly, by performing a global statistical analysis integrating proteomics and transcriptomics. Transcriptomic analysis distinguished 764 differentially expressed genes and revealed that IgG from dcSSc can induce extracellular matrix (ECM) remodeling changes in gene expression profiles in FB. Global statistical analysis integrating proteomics and transcriptomics confirmed that IgG from SSc can induce ECM remodeling and activate FB profiles. This effect depended on the serotype of the patient, suggesting that SSc Aab might play a pathogenic role in some SSc subsets.Lire moins >

Comité de lecture :

Oui

Audience :

Non spécifiée

Établissement(s) :

Université de Lille
CHU Lille

Collections :

• METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694

Date de dépôt :

2022-12-06T16:05:48Z