Titre :

Synthesis and Complexation Ability of a Novel Bis-(guanidinium)-tetrakis-(beta-cyclodextrin) Dendrimeric Tetrapod as a Potential Gene Delivery (DNA and siRNA) System. Study of Cellular siRNA Transfection

Auteur(s) :

Menuel, Stephane [Auteur]
UCCS Équipe Catalyse Supramoléculaire
Fontanay, S. [Auteur]
Clarot, I. [Auteur]
Duval, R. E. [Auteur]
Diez, L. [Auteur]
Marsura, A. [Auteur]

Titre de la revue :

Bioconjugate Chem.

Nom court de la revue :

Bioconjugate Chem.

Pagination :

2357-2362

Date de publication :

2008-11-18

ISSN :

1043-1802

Discipline(s) HAL :

Chimie

Résumé en anglais : [en]

The facile synthesis of a novel bis-(guanidinium)-tetrakis-(β-cyclodextrin) tetrapod, the first example of a new host family, was described, and the ability of the cyclodextrin CyD tetrapod to form molecular association ...
Lire la suite >The facile synthesis of a novel bis-(guanidinium)-tetrakis-(β-cyclodextrin) tetrapod, the first example of a new host family, was described, and the ability of the cyclodextrin CyD tetrapod to form molecular association with siRNA and DNA guest molecules was demonstrated. Affinity capillary electrophoresis was used to determine the binding constant with the evaluation of the shift in the electrophoretic mobility μ of injected siRNA when various CyD tetrapod concentrations were added to the run buffer. A significant association constant (Ka =16 000 M−1) was obtained with borate buffer when double-stranded siRNA was primarily opened with the help of temperature. An efficient cellular transfection of siRNA into human embryonic lung fibroblasts was observed by fluorescence microscopy.Lire moins >

Langue :

Anglais

Établissement(s) :

Université de Lille
CNRS
Centrale Lille
ENSCL
Univ. Artois

Collections :

• Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181

Équipe(s) de recherche :

Catalyse et chimie supramoléculaire (CASU)

Date de dépôt :

2023-05-30T18:09:56Z