Title :

Restricted linear least square treatment processing of heteronuclear spectra of biomolecules using the ANAFOR strategy

Author(s) :

Lippens, Guy [Auteur]
Institut Pasteur de Lille
Bodart, Philippe R. [Auteur]
Université de Lille
Unité de Catalyse et Chimie du Solide - UMR 8181 [UCCS]
Taulelle, Françis [Auteur]
Amoureux, Jean-Paul [Auteur]
Université de Lille

Journal title :

Journal of Magnetic Resonance

Volume number :

161

Pages :

174-182

Publication date :

2016-10-15

ISSN :

1090-7807

Keyword(s) :

data processing
resolution enhancement
triple-resonance spectra

English abstract : [en]

We explore the use of a processing procedure based on restricted least square minimization as a tool for reducing the time versus resolution dilemma often encountered for biomolecular multidimensional spectra. Using a 2D ...
Show more >We explore the use of a processing procedure based on restricted least square minimization as a tool for reducing the time versus resolution dilemma often encountered for biomolecular multidimensional spectra. Using a 2D spectrum as a reference, we obtain the necessary input of frequency components and linewidths. Combined even with a limited time evolution in the indirect dimension, the amplitudes of the correlation peaks in all planes of the 3D spectra can be extracted, and can be used to reconstruct the interferograms in the third dimension. Parameters such as number of lines, threshold choice, resolution, lineshape, number of experimental data points and finally signal to noise ratio of the spectrum are examined starting from a triple-resonance HNCA spectrum of ubiquitin.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS
Centrale Lille
ENSCL
Univ. Artois

Collections :

• Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181

Submission date :

2023-05-30T18:41:47Z
2024-04-19T09:09:58Z