Title :

Novel role of the synaptic scaffold protein Dlgap4 in ventricular surface integrity and neuronal migration during cortical development.

Author(s) :

Romero, D. M. [Auteur]
Institut du Fer à Moulin [IFM - Inserm U1270 - SU]
Poirier, K. [Auteur]
Institut Cochin [IC UM3 (UMR 8104 / U1016)]
Belvindrah, R. [Auteur]
Institut de Biologie du Développement de Marseille [IBDM]
Moutkine, I. [Auteur]
Institut du Fer à Moulin [IFM - Inserm U1270 - SU]
Houllier, A. [Auteur]
Institut du Fer à Moulin [IFM - Inserm U1270 - SU]
Lemoing, A. G. [Auteur]
CHU Amiens-Picardie
Petit, Florence [Auteur]
Maladies Rares du Développement : Génétique, Régulation et Protéomique (RADEME) - ULR 7364
Boland, A. [Auteur]
Institut de Biologie François JACOB [JACOB]
Collins, S. C. [Auteur]
Lipides - Nutrition - Cancer [Dijon - U1231] [LNC]
Soiza-Reilly, M. [Auteur]
Institut du Fer à Moulin
Yalcin, B. [Auteur]
Lipides - Nutrition - Cancer (U866) [LNC]
Chelly, J. [Auteur]
Institut de Génétique et de Biologie Moléculaire et Cellulaire [IGBMC]
Deleuze, J. F. [Auteur]
Institut de Biologie François JACOB [JACOB]
Bahi-Buisson, N. [Auteur]
Institut Cochin [UMR_S567 / UMR 8104]
Francis, F. [Auteur]
Institut du Fer à Moulin [IFM - Inserm U1270 - SU]

Journal title :

Nature Communications

Abbreviated title :

Nat. Commun.

Volume number :

13

Pages :

2746

Publisher :

Nature Publishing Group

Publication date :

2022-05-18

ISSN :

2041-1723

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Subcortical heterotopias are malformations associated with epilepsy and intellectual disability, characterized by the presence of ectopic neurons in the white matter. Mouse and human heterotopia mutations were identified ...
Show more >Subcortical heterotopias are malformations associated with epilepsy and intellectual disability, characterized by the presence of ectopic neurons in the white matter. Mouse and human heterotopia mutations were identified in the microtubule-binding protein Echinoderm microtubule-associated protein-like 1, EML1. Further exploring pathological mechanisms, we identified a patient with an EML1-like phenotype and a novel genetic variation in DLGAP4. The protein belongs to a membrane-associated guanylate kinase family known to function in glutamate synapses. We showed that DLGAP4 is strongly expressed in the mouse ventricular zone (VZ) from early corticogenesis, and interacts with key VZ proteins including EML1. In utero electroporation of Dlgap4 knockdown (KD) and overexpression constructs revealed a ventricular surface phenotype including changes in progenitor cell dynamics, morphology, proliferation and neuronal migration defects. The Dlgap4 KD phenotype was rescued by wild-type but not mutant DLGAP4. Dlgap4 is required for the organization of radial glial cell adherens junction components and actin cytoskeleton dynamics at the apical domain, as well as during neuronal migration. Finally, Dlgap4 heterozygous knockout (KO) mice also show developmental defects in the dorsal telencephalon. We hence identify a synapse-related scaffold protein with pleiotropic functions, influencing the integrity of the developing cerebral cortex.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CHU Lille

Collections :

• Maladies Rares du Développement : Génétique, Régulation et Protéomique (RADEME) - ULR 7364

Submission date :

2023-06-05T07:03:18Z
2023-09-20T08:20:01Z