Autophagy Protects against Eosinophil ...
Type de document :
Article dans une revue scientifique: Article original
DOI :
PMID :
URL permanente :
Titre :
Autophagy Protects against Eosinophil Cytolysis and Release of DNA.
Auteur(s) :
Esnault, Stéphane [Auteur]
University of Wisconsin-Madison
University of Wisconsin School of Medicine and Public Health
Fichtinger, Paul S [Auteur]
Barretto, Karina T [Auteur]
Fogerty, Frances J [Auteur]
Bernau, Ksenija [Auteur]
Mosher, Deane F [Auteur]
Mathur, Sameer K [Auteur]
Sandbo, Nathan [Auteur]
Jarjour, Nizar N [Auteur]
University of Wisconsin-Madison
University of Wisconsin-Madison
University of Wisconsin School of Medicine and Public Health
Fichtinger, Paul S [Auteur]
Barretto, Karina T [Auteur]
Fogerty, Frances J [Auteur]
Bernau, Ksenija [Auteur]
Mosher, Deane F [Auteur]
Mathur, Sameer K [Auteur]
Sandbo, Nathan [Auteur]
Jarjour, Nizar N [Auteur]
University of Wisconsin-Madison
Titre de la revue :
Cells
Nom court de la revue :
Cells
Numéro :
11
Pagination :
1821
Éditeur :
MDPI
Date de publication :
2022-06-02
ISSN :
2073-4409
Mot(s)-clé(s) en anglais :
Autophagy
DNA
Eosinophils
Immunoglobulin G
Interleukin-5
DNA traps
IL3
IL5
IgG
MAP1LC3B
SQSTM1
autophagy
cytolysis
eosinophils
DNA
Eosinophils
Immunoglobulin G
Interleukin-5
DNA traps
IL3
IL5
IgG
MAP1LC3B
SQSTM1
autophagy
cytolysis
eosinophils
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
The presence of eosinophils in the airway is associated with asthma severity and risk of exacerbations. Eosinophils deposit their damaging products in airway tissue, likely by degranulation and cytolysis. We previously ...
Lire la suite >The presence of eosinophils in the airway is associated with asthma severity and risk of exacerbations. Eosinophils deposit their damaging products in airway tissue, likely by degranulation and cytolysis. We previously showed that priming blood eosinophils with IL3 strongly increased their cytolysis on aggregated IgG. Conversely, IL5 priming did not result in significant eosinophil cytolysis in the same condition. Therefore, to identify critical events protecting eosinophils from cell cytolysis, we examined the differential intracellular events between IL5- and IL3-primed eosinophils interacting with IgG. We showed that both IL3 and IL5 priming increased the eosinophil adhesion to IgG, phosphorylation of p38, and production of reactive oxygen species (ROS), and decreased the phosphorylation of cofilin. However, autophagic flux as measured by the quantification of SQSTM1-p62 and lipidated-MAP1L3CB over time on IgG, with or without bafilomycin-A1, was higher in IL5-primed compared to IL3-primed eosinophils. In addition, treatment with bafilomycin-A1, an inhibitor of granule acidification and autophagolysosome formation, enhanced eosinophil cytolysis and DNA trap formation in IL5-primed eosinophils. Therefore, this study suggests that increased autophagy in eosinophils protects from cytolysis and the release of DNA, and thus limits the discharge of damaging intracellular eosinophilic contents.Lire moins >
Lire la suite >The presence of eosinophils in the airway is associated with asthma severity and risk of exacerbations. Eosinophils deposit their damaging products in airway tissue, likely by degranulation and cytolysis. We previously showed that priming blood eosinophils with IL3 strongly increased their cytolysis on aggregated IgG. Conversely, IL5 priming did not result in significant eosinophil cytolysis in the same condition. Therefore, to identify critical events protecting eosinophils from cell cytolysis, we examined the differential intracellular events between IL5- and IL3-primed eosinophils interacting with IgG. We showed that both IL3 and IL5 priming increased the eosinophil adhesion to IgG, phosphorylation of p38, and production of reactive oxygen species (ROS), and decreased the phosphorylation of cofilin. However, autophagic flux as measured by the quantification of SQSTM1-p62 and lipidated-MAP1L3CB over time on IgG, with or without bafilomycin-A1, was higher in IL5-primed compared to IL3-primed eosinophils. In addition, treatment with bafilomycin-A1, an inhibitor of granule acidification and autophagolysosome formation, enhanced eosinophil cytolysis and DNA trap formation in IL5-primed eosinophils. Therefore, this study suggests that increased autophagy in eosinophils protects from cytolysis and the release of DNA, and thus limits the discharge of damaging intracellular eosinophilic contents.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
Université de Lille
Inserm
CHU Lille
Inserm
CHU Lille
Collections :
Date de dépôt :
2023-10-18T11:36:55Z
2023-10-25T09:18:55Z
2023-10-25T09:18:55Z
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