Potent synergistic effect of IL-3 and TNF ...
Type de document :
Article dans une revue scientifique: Article original
PMID :
URL permanente :
Titre :
Potent synergistic effect of IL-3 and TNF on matrix metalloproteinase 9 generation by human eosinophils.
Auteur(s) :
Kelly, Elizabeth A B [Auteur]
University of Wisconsin School of Medicine and Public Health
Liu, Lin Ying [Auteur]
University of Wisconsin School of Medicine and Public Health
Esnault, Stéphane [Auteur]
University of Wisconsin School of Medicine and Public Health
Quinchia Johnson, Beatriz Helena [Auteur]
University of Wisconsin School of Medicine and Public Health
Jarjour, Nizar N [Auteur]
University of Wisconsin School of Medicine and Public Health
University of Wisconsin School of Medicine and Public Health
Liu, Lin Ying [Auteur]
University of Wisconsin School of Medicine and Public Health
Esnault, Stéphane [Auteur]
University of Wisconsin School of Medicine and Public Health
Quinchia Johnson, Beatriz Helena [Auteur]
University of Wisconsin School of Medicine and Public Health
Jarjour, Nizar N [Auteur]
University of Wisconsin School of Medicine and Public Health
Titre de la revue :
Cytokine
Numéro :
58
Pagination :
199-206
Date de publication :
2012-05-01
ISSN :
1096-0023
Mot(s)-clé(s) en anglais :
Cytokines
Enzyme-Linked Immunosorbent Assay
Eosinophils
Extracellular Signal-Regulated MAP Kinases
Flow Cytometry
Humans
Interleukin-3
Matrix Metalloproteinase 9
Phosphorylation
RNA, Messenger
Real-Time Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Enzyme-Linked Immunosorbent Assay
Eosinophils
Extracellular Signal-Regulated MAP Kinases
Flow Cytometry
Humans
Interleukin-3
Matrix Metalloproteinase 9
Phosphorylation
RNA, Messenger
Real-Time Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
TNF (designated as TNF-α under previous nomenclature) is the preeminent activator of MMP-9 generation from a variety of cells including eosinophils. We have previously established that TNF strongly synergizes with IFN-γ ...
Lire la suite >TNF (designated as TNF-α under previous nomenclature) is the preeminent activator of MMP-9 generation from a variety of cells including eosinophils. We have previously established that TNF strongly synergizes with IFN-γ and IL-4 for eosinophil synthesis of Th1- and Th2-type chemokines respectively. Thus, we sought to determine if TNF-induced synthesis of MMP-9 would be enhanced by the presence of Th1, Th2, or the eosinophil-associated common beta chain (βc) cytokines. Human blood eosinophils were cultured with TNF alone or in combination with either IFN-γ, IL-4, IL-3, IL-5, or GM-CSF. Concentrations and activities of MMP-9 in eosinophil culture supernates were measured by ELISA and gelatin zymography, mRNA transcription and stabilization by quantitative real-time PCR, and signaling events by immunoblotting and intracellular flow cytometric analysis. Individually, TNF, GM-CSF, or IL-3, but not IL-4 or IFN-γ, induced relatively small (GM-CSF>IL-5. Zymography revealed that eosinophils release MMP-9 in its pro-form. Eosinophil stimulation with the combination of IL-3 plus TNF led to increased steady-state levels of MMP-9 mRNA, prolonged mRNA stabilization, and enhanced activation of ERK1/2 phosphorylation. Inhibition of NF-κB, MEK kinase, or p38 MAP kinase, but not JNK signaling pathways, diminished IL-3/TNF-induced MMP-9 mRNA and protein production. Thus, the synergistic regulation of eosinophil MMP-9 by IL-3 plus TNF likely involves cooperative interaction of multiple transcription factors downstream from ERK, p38, and NF-κB activation as well as post-transcriptional regulation of MMP-9 mRNA stabilization. Our data indicate that within microenvironments rich in βc-family cytokines and TNF, eosinophils are an important source of proMMP-9 and highlight a previously unrecognized role for synergistic interaction between TNF and βc-family cytokines, particularly IL-3, for proMMP-9 synthesis.Lire moins >
Lire la suite >TNF (designated as TNF-α under previous nomenclature) is the preeminent activator of MMP-9 generation from a variety of cells including eosinophils. We have previously established that TNF strongly synergizes with IFN-γ and IL-4 for eosinophil synthesis of Th1- and Th2-type chemokines respectively. Thus, we sought to determine if TNF-induced synthesis of MMP-9 would be enhanced by the presence of Th1, Th2, or the eosinophil-associated common beta chain (βc) cytokines. Human blood eosinophils were cultured with TNF alone or in combination with either IFN-γ, IL-4, IL-3, IL-5, or GM-CSF. Concentrations and activities of MMP-9 in eosinophil culture supernates were measured by ELISA and gelatin zymography, mRNA transcription and stabilization by quantitative real-time PCR, and signaling events by immunoblotting and intracellular flow cytometric analysis. Individually, TNF, GM-CSF, or IL-3, but not IL-4 or IFN-γ, induced relatively small (GM-CSF>IL-5. Zymography revealed that eosinophils release MMP-9 in its pro-form. Eosinophil stimulation with the combination of IL-3 plus TNF led to increased steady-state levels of MMP-9 mRNA, prolonged mRNA stabilization, and enhanced activation of ERK1/2 phosphorylation. Inhibition of NF-κB, MEK kinase, or p38 MAP kinase, but not JNK signaling pathways, diminished IL-3/TNF-induced MMP-9 mRNA and protein production. Thus, the synergistic regulation of eosinophil MMP-9 by IL-3 plus TNF likely involves cooperative interaction of multiple transcription factors downstream from ERK, p38, and NF-κB activation as well as post-transcriptional regulation of MMP-9 mRNA stabilization. Our data indicate that within microenvironments rich in βc-family cytokines and TNF, eosinophils are an important source of proMMP-9 and highlight a previously unrecognized role for synergistic interaction between TNF and βc-family cytokines, particularly IL-3, for proMMP-9 synthesis.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
Université de Lille
Inserm
CHU Lille
Inserm
CHU Lille
Collections :
Date de dépôt :
2023-11-14T10:40:50Z
2024-03-18T09:01:11Z
2024-03-18T09:01:11Z
Fichiers
- IL3 TNF MMP9 2012.pdf
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