Titre :

GEMC1 and MCIDAS interactions with SWI/SNF complexes regulate the multiciliated cell-specific transcriptional program.

Auteur(s) :

Lewis, M. [Auteur]
Terré, B. [Auteur]
Knobel, P. A. [Auteur]
Cheng, T. [Auteur]
Lu, H. [Auteur]
Attolini, C. S. [Auteur]
Smak, J. [Auteur]
Coyaud, Etienne-Marie [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Garcia-Cao, I. [Auteur]
Sharma, S. [Auteur]
Vineethakumari, C. [Auteur]
Querol, J. [Auteur]
Gil-Gómez, G. [Auteur]
Piergiovanni, G. [Auteur]
Costanzo, V. [Auteur]
Peiró, S. [Auteur]
Raught, B. [Auteur]
Zhao, H. [Auteur]
Salvatella, X. [Auteur]
Roy, S. [Auteur]
Mahjoub, M. R. [Auteur]
Stracker, T. H. [Auteur]

Titre de la revue :

Cell Death and Disease

Nom court de la revue :

Cell Death Dis

Numéro :

14

Pagination :

201

Date de publication :

2023-03-19

ISSN :

2041-4889

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Multiciliated cells (MCCs) project dozens to hundreds of motile cilia from their apical surface to promote the movement of fluids or gametes in the mammalian brain, airway or reproductive organs. Differentiation of MCCs ...
Lire la suite >Multiciliated cells (MCCs) project dozens to hundreds of motile cilia from their apical surface to promote the movement of fluids or gametes in the mammalian brain, airway or reproductive organs. Differentiation of MCCs requires the sequential action of the Geminin family transcriptional activators, GEMC1 and MCIDAS, that both interact with E2F4/5-DP1. How these factors activate transcription and the extent to which they play redundant functions remains poorly understood. Here, we demonstrate that the transcriptional targets and proximal proteomes of GEMC1 and MCIDAS are highly similar. However, we identified distinct interactions with SWI/SNF subcomplexes; GEMC1 interacts primarily with the ARID1A containing BAF complex while MCIDAS interacts primarily with BRD9 containing ncBAF complexes. Treatment with a BRD9 inhibitor impaired MCIDAS-mediated activation of several target genes and compromised the MCC differentiation program in multiple cell based models. Our data suggest that the differential engagement of distinct SWI/SNF subcomplexes by GEMC1 and MCIDAS is required for MCC-specific transcriptional regulation and mediated by their distinct C-terminal domains.Lire moins >

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
Inserm
CHU Lille

Collections :

• Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Date de dépôt :

2023-12-13T03:48:00Z
2024-01-26T12:30:27Z