Starch-based NP act as antigen delivery ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Permalink :
Title :
Starch-based NP act as antigen delivery systems without immunomodulating effect.
Author(s) :
Fasquelle, Francois [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Dubuquoy, Laurent [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Betbeder, Didier [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Dubuquoy, Laurent [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Betbeder, Didier [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Journal title :
PLoS ONE
Abbreviated title :
PLoS One
Volume number :
17
Pages :
e0272234
Publication date :
2023-04-04
ISSN :
1932-6203
English keyword(s) :
Antigen encapsulation
Epithelial cells
Secretion
Immune response
Antigens
Cytokines
Endocytosis
Nanoparticles
Epithelial cells
Secretion
Immune response
Antigens
Cytokines
Endocytosis
Nanoparticles
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
The nasal route of immunization has become a real alternative to injections. It is indeed described as more efficient at inducing immune protection, since it initiates both mucosal and systemic immunity, thus protecting ...
Show more >The nasal route of immunization has become a real alternative to injections. It is indeed described as more efficient at inducing immune protection, since it initiates both mucosal and systemic immunity, thus protecting against both the infection itself and the transmission of pathogens by the host. However, the use of immunomodulators should be limited since they induce inflammation. Here we investigated in vitro the mechanisms underlying the enhancement of antigen immunogenicity by starch nanoparticles (NPL) delivery systems in H292 epithelial cells, as well as the NPL’s immunomodulatory effect. We observed that NPL had no intrinsic immunomodulatory effect but enhanced the immunogenicity of an E. coli lysate (Ag) merely by increasing its intracellular delivery. Moreover, we demonstrated the importance of the NPL density on their efficiency by comparing reticulated (NPL) and non-reticulated particles (NPL·NR). These results show that an efficient delivery system is sufficient to induce a mucosal immune response without the use of immunomodulators.Show less >
Show more >The nasal route of immunization has become a real alternative to injections. It is indeed described as more efficient at inducing immune protection, since it initiates both mucosal and systemic immunity, thus protecting against both the infection itself and the transmission of pathogens by the host. However, the use of immunomodulators should be limited since they induce inflammation. Here we investigated in vitro the mechanisms underlying the enhancement of antigen immunogenicity by starch nanoparticles (NPL) delivery systems in H292 epithelial cells, as well as the NPL’s immunomodulatory effect. We observed that NPL had no intrinsic immunomodulatory effect but enhanced the immunogenicity of an E. coli lysate (Ag) merely by increasing its intracellular delivery. Moreover, we demonstrated the importance of the NPL density on their efficiency by comparing reticulated (NPL) and non-reticulated particles (NPL·NR). These results show that an efficient delivery system is sufficient to induce a mucosal immune response without the use of immunomodulators.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
Inserm
CHU Lille
Inserm
CHU Lille
Submission date :
2024-01-12T00:58:07Z
2024-01-25T08:53:36Z
2024-01-25T08:53:36Z
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