Titre :

Faster and less invasive tools to identify patients with ileal colonization by adherent-invasive E. coli in Crohn's disease.

Auteur(s) :

Buisson, Anthony [Auteur]
Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte [M2iSH]
Service Hépato-Gastro-Entérologie [CHU Clermont-Ferrand]
Infection Inflammation et Interaction Hôtes Pathogènes [CHU Clermont-Ferrand] [3IHP ]
CHU Clermont-Ferrand
Vazeille, E. [Auteur]
Fumery, Mathurin [Auteur]
Périnatalité et Risques Toxiques - UMR INERIS_I 1 UPJV [PERITOX]
Service d'Hépato Gastroenterologie [CHU Amiens-Picardie]
Pariente, Benjamin [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Nancey, Stéphane [Auteur]
Hospices Civils de Lyon [HCL]
Centre International de Recherche en Infectiologie [CIRI]
Seksik, Philippe [Auteur]
CHU Saint-Antoine [AP-HP]
Peyrin-Biroulet, Laurent [Auteur]
Centre Hospitalier Régional Universitaire de Nancy [CHRU Nancy]
Nutrition-Génétique et Exposition aux Risques Environnementaux [NGERE]
Allez, Matthieu [Auteur]
Hopital Saint-Louis [AP-HP] [AP-HP]
Ecotaxie, microenvironnement et développement lymphocytaire [EMily (UMR_S_1160 / U1160)]
Ballet, N. [Auteur]
Filippi, J. [Auteur]
Yzet, C. [Auteur]
Nachury, Maria [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Boschetti, G. [Auteur]
Billard, E. [Auteur]
Dubois, A. [Auteur]
Rodriguez, S. [Auteur]
Chevarin, C. [Auteur]
Goutte, M. [Auteur]
Bommelaer, G. [Auteur]
Pereira, Bruno [Auteur]
Service Biostatistiques, Télématiques, Traitement de l’image [CHU Clermont-Ferrand]
CHU Clermont-Ferrand
Direction de la Recherche Clinique et de l’Innovation [CHU Clermont-Ferrand] [DRCI]
Hebuterne, X. [Auteur]
Barnich, N. [Auteur]

Titre de la revue :

United European Gastroenterology Journal

Nom court de la revue :

United European Gastroenterol J

Numéro :

9

Pagination :

1007-1018

Date de publication :

2021-11-24

ISSN :

2050-6414

Mot(s)-clé(s) en anglais :

adherent-invasive E
coli
anti-E
coli antibodies
CEACAM6
IBD
Crohn's disease
inflammatory bowel disease

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Background and Aims The identification of Crohn's disease (CD)-associated adherent and invasive Escherichia coli (AIEC) is time-consuming and requires ileal biopsies. We aimed to identify a faster and less invasive methods ...
Lire la suite >Background and Aims The identification of Crohn's disease (CD)-associated adherent and invasive Escherichia coli (AIEC) is time-consuming and requires ileal biopsies. We aimed to identify a faster and less invasive methods to detect ileal colonization by AIEC in CD patients. Methods CD patients requiring ileo-colonoscopy were consecutively enrolled in this prospective multicenter study. Samples from saliva, serum, stools, and ileal biopsies of CD patients were collected. Results Among 102 CD patients, the prevalence of AIEC on ileal biopsies was 24.5%. The abundance and global invasive ability of ileal-associated total E. coli were respectively ten-fold (p = 0.0065) and two-fold (p = 0.0007) higher in AIEC-positive (vs. AIEC-negative), while abundance of total E. coli in the feces was not correlated with AIEC status in the ileum. The best threshold of ileal total E. coli was 60 cfu/biopsy to detect AIEC-positive patients, with high negative predictive value (NPV) (94.1%[80.3–99.3]), while the global invasive ability (>9000 internalized bacteria) was able to detect the presence of AIEC with high positive predictive value (80.0% [55.2–100.0]). Overall, 78.1% of the AIEC + patients were colonized by two or less different AIEC strains. The level of serum anti-total E. coli antibodies (AEcAb) was higher in AIEC-positive patients (p = 0.038) with a very high negative predictive value (96.6% [89.9–100.0]) (p = 0.038) for a cut-off value > 1.9 × 10−3. Conclusions More than two thirds of AIEC-positive CD patients were colonized by two or less AIEC strains. While stools samples are not accurate to screen AIEC status, the AEcAb level appears to be an attractive, rapid and easier biomarker to identify patients with Crohn's disease harboring AIEC.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
Inserm
CHU Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Date de dépôt :

2024-01-12T05:39:11Z
2024-02-27T15:32:57Z