Isolation and structure elucidation of ...
Type de document :
Article dans une revue scientifique: Article original
PMID :
URL permanente :
Titre :
Isolation and structure elucidation of cell surface polysaccharides from Oenococcus oeni.
Auteur(s) :
Maes, Emmanuel [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Sadovskaya, Irina [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Yamakawa, Nao [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Goulet, A. [Auteur]
Le Marrec, C. [Auteur]
Chapot-Chartier, M. P. [Auteur]

Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Sadovskaya, Irina [Auteur]

BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Yamakawa, Nao [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Goulet, A. [Auteur]
Le Marrec, C. [Auteur]
Chapot-Chartier, M. P. [Auteur]
Titre de la revue :
Carbohydrate Research
Nom court de la revue :
Carbohydr Res
Numéro :
552
Pagination :
109456
Éditeur :
Elsevier
Date de publication :
2025-03-31
ISSN :
1873-426X
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
The Gram-positive bacterium Oenococcus oeni is a major player in wine malolactic fermentation. In O. oeni, cell wall polysaccharides are considered putative receptors for bacteriophages, virus predators that lead to ...
Lire la suite >The Gram-positive bacterium Oenococcus oeni is a major player in wine malolactic fermentation. In O. oeni, cell wall polysaccharides are considered putative receptors for bacteriophages, virus predators that lead to fermentation failures. In this study, we have developed an efficient stepwise extraction protocol to extract polysaccharides from the cell wall of O. oeni IOEBS277, which were analyzed by methylation, 1D, 2D-NMR spectroscopy, and MALDI-QIT-TOF mass spectrometry. The chemical structures of the two major purified polysaccharides were elucidated. The first one is a heteropolysaccharide with repeating units consisting of a branched hexasaccharide and one glycerol residue, linked by phosphodiester bonds. The second one consists of a →6)-β-Galf-(1→ galactofuranan chain partially substituted on the C-2 hydroxyl with β-Glcp. HR-MAS NMR analysis of intact O. oeni cells indicated that both polysaccharides are exposed to the bacterial surface.Lire moins >
Lire la suite >The Gram-positive bacterium Oenococcus oeni is a major player in wine malolactic fermentation. In O. oeni, cell wall polysaccharides are considered putative receptors for bacteriophages, virus predators that lead to fermentation failures. In this study, we have developed an efficient stepwise extraction protocol to extract polysaccharides from the cell wall of O. oeni IOEBS277, which were analyzed by methylation, 1D, 2D-NMR spectroscopy, and MALDI-QIT-TOF mass spectrometry. The chemical structures of the two major purified polysaccharides were elucidated. The first one is a heteropolysaccharide with repeating units consisting of a branched hexasaccharide and one glycerol residue, linked by phosphodiester bonds. The second one consists of a →6)-β-Galf-(1→ galactofuranan chain partially substituted on the C-2 hydroxyl with β-Glcp. HR-MAS NMR analysis of intact O. oeni cells indicated that both polysaccharides are exposed to the bacterial surface.Lire moins >
Langue :
Anglais
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
Université de Lille
CHU Lille
CNRS
Inserm
Institut Pasteur de Lille
CHU Lille
CNRS
Inserm
Institut Pasteur de Lille
Date de dépôt :
2025-04-01T21:03:38Z
2025-04-09T07:42:59Z
2025-04-09T07:42:59Z