Protein recycling and limb muscle recovery after critical illness in slow- and fast-twitch limb muscle.

Preau, Sebastien; Ambler, Michael; Sigurta, Anna; Kleyman, Anna; Dyson, Alex; Hill, Neil Edward; Boulanger, Eric; Singer, Mervyn

Type de document :

Article dans une revue scientifique: Article original

DOI :

10.1152/ajpregu.00221.2018

PMID :

30789789

URL permanente :

http://hdl.handle.net/20.500.12210/13777

Titre :

Protein recycling and limb muscle recovery after critical illness in slow- and fast-twitch limb muscle.

Auteur(s) :

Preau, Sebastien [Auteur]

Lille Inflammation Research International Center (LIRIC) - U995
Lille Inflammation Research International Center - U 995 [LIRIC]
Ambler, Michael [Auteur]
Sigurta, Anna [Auteur]
Kleyman, Anna [Auteur]
Dyson, Alex [Auteur]
Hill, Neil Edward [Auteur]
Boulanger, Eric [Auteur]

Lille Inflammation Research International Center (LIRIC) - U995
Lille Inflammation Research International Center - U 995 [LIRIC]
Singer, Mervyn [Auteur]

Titre de la revue :

American journal of physiology. Regulatory, integrative and comparative physiology

Nom court de la revue :

Am. J. Physiol. Regul. Integr. Comp. Physiol.

Date de publication :

2019-02-21

ISSN :

1522-1490

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

An impaired capacity of muscle to regenerate after critical illness results in long-term functional disability. We previously described in a long-term rat peritonitis model that gastrocnemius displays near-normal histology ...
Lire la suite >An impaired capacity of muscle to regenerate after critical illness results in long-term functional disability. We previously described in a long-term rat peritonitis model that gastrocnemius displays near-normal histology whereas soleus demonstrates a necrotizing phenotype. We thus investigated the link between the necrotizing phenotype of critical illness myopathy and proteasome activity in these two limb muscles. We studied male Wistar rats that underwent an intraperitoneal injection of the fungal cell wall constituent zymosan or n-saline as a sham-treated control. Rats ( = 74) were killed at 2, 7, and 14 days postintervention with gastrocnemius and soleus muscle removed and studied ex vivo. Zymosan-treated animals displayed an initial reduction of body weight but a persistent decrease in mass of both lower hindlimb muscles. Zymosan increased chymotrypsin- and trypsin-like proteasome activities in gastrocnemius at and but in soleus at only. Activated caspases-3 and -9, polyubiquitin proteins, and 14-kDa fragments of myofibrillar actin (proteasome substrates) remained persistently increased from to in soleus but not in gastrocnemius. These results suggest that a relative proteasome deficiency in soleus is associated with a necrotizing phenotype during long-term critical illness. Rescuing proteasome clearance may offer a potential therapeutic option to prevent long-term functional disability in critically ill patients.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

CHU Lille
Inserm
Université de Lille

Collections :

Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Date de dépôt :

2019-10-22T08:09:09Z

Numéro de version	Lien	Date de modification
2	20.500.12210/13777	2024-03-22T09:23:07Z
1	20.500.12210/13777.1*	2019-10-22T08:09:09Z

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Protein recycling and limb muscle recovery ...

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