Title :

Toward the standardization of mycological examination of sputum samples in cystic fibrosis: results from a french multicenter prospective study

Author(s) :

Coron, Noemie [Auteur]
Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
Pihet, Marc [Auteur]
Frealle, émilie [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Lemeille, Yolande [Auteur]
Université Grenoble Alpes [2016-2019] [UGA [2016-2019]]
Pinel, Claudine [Auteur]
Université Grenoble Alpes [2016-2019] [UGA [2016-2019]]
Pelloux, Herve [Auteur]
Université Grenoble Alpes [2016-2019] [UGA [2016-2019]]
Gargala, Gilles [Auteur]
Favennec, Loic [Auteur]
Accoceberry, Isabelle [Auteur]
Université de Bordeaux [UB]
Durand-Joly, Isabelle [Auteur]
Dalle, Frédéric [Auteur]
Huet, Frédéric [Auteur]
Fanton, Annlyse [Auteur]
Boldron, Amale [Auteur]
Loeuille, Guy-Andre [Auteur]
Domblides, Philippe [Auteur]
Coltey, Berengere [Auteur]
Pin, Isabelle [Auteur]
Llerena, Catherine [Auteur]
Troussier, Françoise [Auteur]
Person, Christine [Auteur]
Marguet, Christophe [Auteur]
Wizla, Nathalie [Auteur]
Université Lille Nord (France)
Thumerelle, Caroline [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Turck, Dominique [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Bui, Stephanie [Auteur]
Université de Bordeaux [UB]
Fayon, Michael [Auteur]
Université de Bordeaux [UB]
Duhamel, Alain [Auteur]
Evaluation des technologies de santé et des pratiques médicales - ULR 2694 [METRICS]
Prevotat, Anne [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Wallaert, Benoit [Auteur]
Université Lille Nord (France)
Leroy, Sylvie [Auteur]
Université Lille Nord (France)
Bouchara, Jean-Philippe [Auteur]
Delhaes, Laurence [Auteur]
Université Lille Nord (France)

Journal title :

Mycopathologia

Abbreviated title :

Mycopathologia

Volume number :

183

Pages :

101-117

Publication date :

2018-02

ISSN :

0301-486X

English keyword(s) :

Molds
Cystic fibrosis
Mycological examination
Filamentous fungi
Lung mycobiota
Aspergillus

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Fungal respiratory colonization of cystic fibrosis (CF) patients emerges as a new concern; however, the heterogeneity of mycological protocols limits investigations. We first aimed at setting up an efficient standardized ...
Show more >Fungal respiratory colonization of cystic fibrosis (CF) patients emerges as a new concern; however, the heterogeneity of mycological protocols limits investigations. We first aimed at setting up an efficient standardized protocol for mycological analysis of CF sputa that was assessed during a prospective, multicenter study: "MucoFong" program (PHRC-06/1902). Sputa from 243 CF patients from seven centers in France were collected over a 15-month period and submitted to a standardized protocol based on 6 semi-selective media. After mucolytic pretreatment, sputa were plated in parallel on cycloheximide-enriched (ACT37), erythritol-enriched (ERY37), benomyl dichloran-rose bengal (BENO37) and chromogenic (CAN37) media incubated at 37 °C and on Sabouraud-chloramphenicol (SAB27) and erythritol-enriched (ERY27) media incubated at 20-27 °C. Each plate was checked twice a week during 3 weeks. Fungi were conventionally identified; time for detection of fungal growth was noted for each species. Fungal prevalences and media performances were assessed; an optimal combination of media was determined using the Chi-squared automatic interaction detector method. At least one fungal species was isolated from 81% of sputa. Candida albicans was the most prevalent species (58.8%), followed by Aspergillus fumigatus (35.4%). Cultivation on CAN37, SAB27, ACT37 and ERY27 during 16 days provided an optimal combination, detecting C. albicans, A. fumigatus, Scedosporium apiospermum complex and Exophiala spp. with sensitivities of 96.5, 98.8, 100 and 100%. Combination of these four culture media is recommended to ensure the growth of key fungal pathogens in CF respiratory specimens. The use of such consensual protocol is of major interest for merging results from future epidemiological studies.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
CNRS
Inserm
Institut Pasteur de Lille
Université de Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
• METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694

Submission date :

2020-02-11T09:07:35Z
2024-01-15T12:12:41Z