Addition of regular insulin to ternary parenteral nutrition: a stability study

HENRY, Héloïse; LANNOY, Damien; Maboudou, Patrice; Seguy, David; dine, thierry; Pigny, Pascal; Odou, Pascal

Document type :

Article dans une revue scientifique: Article original

DOI :

10.3390/pharmaceutics13040458

PMID :

33801784

Permalink :

http://hdl.handle.net/20.500.12210/40865

Title :

Addition of regular insulin to ternary parenteral nutrition: a stability study

Author(s) :

HENRY, Héloïse [Auteur]

Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - ULR 7365
LANNOY, Damien [Auteur]

Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - ULR 7365
Maboudou, Patrice [Auteur]
Seguy, David [Auteur]

Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
dine, thierry [Auteur]

Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - ULR 7365
Pigny, Pascal [Auteur]

Miniaturisation pour la Synthèse, l'Analyse et la Protéomique (MSAP) - USR 3290
Odou, Pascal [Auteur]

Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - ULR 7365

Journal title :

Pharmaceutics

Abbreviated title :

Pharmaceutics

Volume number :

Publication date :

2021-03-27

ISSN :

1999-4923

Keyword(s) :

parenteral nutrition solutions
stability
immunoassay
regular insulin

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

BACKGROUND: Parenteral nutrition (PN) is a complex medium in which added insulin can become unstable. The aim of this study is, therefore, to evaluate the stability of insulin in PN and to identify influencing factors. METHODS: ...
Show more >BACKGROUND: Parenteral nutrition (PN) is a complex medium in which added insulin can become unstable. The aim of this study is, therefore, to evaluate the stability of insulin in PN and to identify influencing factors. METHODS: A total of 20 IU/L of regular insulin was added to PN in either glass or Ethylene Vinyl Acetate (EVA) containers. A 24 h stability study was performed via an electrochemiluminescence immunoassay in different media: A ternary PN admixture, separate compartments of the PN bag and a binary admixture. This study was repeated in the absence of zinc, with the addition of serum albumin or tween and with pH adjustment (3.6 or 6.3). Insulin concentration at t time was expressed as a percentage of the initial insulin concentration. Analysis of covariance (ANCOVA) was applied to determine the factors that influence insulin stability. RESULTS: In all PN admixtures, the insulin concentration ratio decreased, stabilising at a 60% and then plateauing after 6 h. At pH 3.6, the ratio was above 90%, while at pH 6.3 it decreased, except in the amino acid solution. ANCOVA (r2p CONCLUSIONS: A low pH level seems to stabilise insulin in PN admixtures. The influence of dextrose content suggests that insulin glycation may influence stability.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
CNRS
Inserm
Université de Lille

Collections :

Submission date :

2021-07-06T12:50:40Z

Version	Link	Modification date
3	20.500.12210/40865	2022-11-09T09:58:42Z
2	20.500.12210/40865.2	2022-01-19T08:47:56Z
1	20.500.12210/40865.1*	2021-07-06T12:50:40Z

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Addition of regular insulin to ternary ...

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