Title :

Dipalmitoyl-phosphatidylserine-filled cationic maltodextrin nanoparticles exhibit enhanced efficacy for cell entry and intracellular protein delivery in phagocytic THP-1 cells.

Author(s) :

Brinkhuizen, Clément [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Shapman, Damien [Auteur]
Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie [PRIMACEN]
High-tech Research Infrastructures for Life Sciences [HeRacLeS]
Lebon, A. [Auteur]
Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie [PRIMACEN]
Bénard, M. [Auteur]
Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie [PRIMACEN]
Tardivel, Meryem [Auteur]
Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41
Dubuquoy, Laurent [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Galas, L. [Auteur]
Carpentier, Rodolphe [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]

Journal title :

Biomolecular concepts

Abbreviated title :

Biomol Concepts

Volume number :

14

Publication date :

2023-06-28

ISSN :

1868-503X

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Vaccination through the upper respiratory tract is a promising strategy, and particulate antigens, such as antigens associated with nanoparticles, triggered a stronger immune response than the sole antigens. Cationic ...
Show more >Vaccination through the upper respiratory tract is a promising strategy, and particulate antigens, such as antigens associated with nanoparticles, triggered a stronger immune response than the sole antigens. Cationic maltodextrin-based nanoparticles loaded with phosphatidylglycerol (NPPG) are efficient for intranasal vaccination but non-specific to trigger immune cells. Here we focused on phosphatidylserine (PS) receptors, specifically expressed by immune cells including macrophages, to improve nanoparticle targeting through an efferocytosis-like mechanism. Consequently, the lipids associated with NPPG have been substituted by PS to generate cationic maltodextrin-based nanoparticles with dipalmitoyl-phosphatidylserine (NPPS). Both NPPS and NPPG exhibited similar physical characteristics and intracellular distribution in THP-1 macrophages. NPPS cell entry was faster and higher (two times more) than NPPG. Surprisingly, competition of PS receptors with phospho-L-serine did not alter NPPS cell entry and annexin V did not preferentially interact with NPPS. Although the protein association is similar, NPPS delivered more proteins than NPPG in cells. On the contrary, the proportion of mobile nanoparticles (50%), the movement speed of nanoparticles (3 µm/5 min), and protein degradation kinetics in THP-1 were not affected by lipid substitution. Together, the results indicate that NPPS enter cells and deliver protein better than NPPG, suggesting that modification of the lipids of cationic maltodextrin-based nanoparticles may be a useful strategy to enhance nanoparticle efficacy for mucosal vaccination.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
• Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41

Submission date :

2023-12-21T06:35:46Z
2024-02-23T11:25:26Z