Proteomic Characterization of Drosophila melanogaster Proboscis

Aruçi, E.; Saliou, Jean-Michel; Ferveur, J. F.; Briand, L.

Type de document :

Article dans une revue scientifique: Article original

DOI :

10.3390/biology11111687

PMID :

36421401

URL permanente :

http://hdl.handle.net/20.500.12210/95729

Titre :

Proteomic Characterization of Drosophila melanogaster Proboscis

Auteur(s) :

Aruçi, E. [Auteur]
Saliou, Jean-Michel [Auteur]

Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41
Ferveur, J. F. [Auteur]
Briand, L. [Auteur]

Titre de la revue :

Biology (Basel)

Nom court de la revue :

Biology (Basel)

Numéro :

Pagination :

p. 1687

Date de publication :

2022

ISSN :

2079-7737

Mot(s)-clé(s) en anglais :

chemosensory system
taste
odorant-binding proteins
mass spectrometry
proteomics

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Drosophila melanogaster flies use their proboscis to taste and distinguish edible compounds from toxic compounds. With their proboscis, flies can detect sex pheromones at a close distance or by contact. Most of the known ...
Lire la suite >Drosophila melanogaster flies use their proboscis to taste and distinguish edible compounds from toxic compounds. With their proboscis, flies can detect sex pheromones at a close distance or by contact. Most of the known proteins associated with probosci’s detection belong to gustatory receptor families. To extend our knowledge of the proboscis-taste proteins involved in chemo-detection, we used a proteomic approach to identify soluble proteins from Drosophila females and males. This investigation, performed with hundreds of dissected proboscises, was initiated by the chromatographic separation of tryptic peptides, followed by tandem mass spectrometry, allowing for femtomole detection sensitivity. We found 586 proteins, including enzymes, that are involved in intermediary metabolism and proteins dedicated to various functions, such as nucleic acid metabolism, ion transport, immunity, digestion, and organ development. Among 60 proteins potentially involved in chemosensory detection, we identified two odorant-binding proteins (OBPs), i.e., OBP56d (which showed much higher expression in females than in males) and OBP19d. Because OBP56d was also reported to be more highly expressed in the antennae of females, this protein can be involved in the detection of both volatile and contact male pheromone(s). Our proteomic study paves the way to better understand the complex role of Drosophila proboscis in the chemical detection of food and pheromonal compounds.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41

Date de dépôt :

2023-12-21T06:45:23Z
2024-02-23T11:42:24Z

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Numéro de version	Lien	Date de modification
2	20.500.12210/95729*	2024-02-23T11:39:03Z
1	20.500.12210/95729.1	2023-12-21T06:45:23Z

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