Measurement of fish freshness: Flow cytometry analysis of isolated muscle mitochondria.

Soret, Meline; Bouchendhomme, T.; Cleach, J.; Jouy, Nathalie; Crola Da Silva, C.; Devin, A.; Grard, Thierry; Lencel, P.

Document type :

Article dans une revue scientifique: Article original

DOI :

10.1016/j.foodchem.2021.131690

PMID :

34865931

Permalink :

http://hdl.handle.net/20.500.12210/95745

Title :

Measurement of fish freshness: Flow cytometry analysis of isolated muscle mitochondria.

Author(s) :

Soret, Meline [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Bouchendhomme, T. [Auteur]
Cleach, J. [Auteur]
Jouy, Nathalie [Auteur]
Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41
Plateforme BioImaging Center Lille - PLBS [BICeL]
Crola Da Silva, C. [Auteur]
Devin, A. [Auteur]
Grard, Thierry [Auteur]

BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Lencel, P. [Auteur]

Journal title :

Food Chemistry: X

Abbreviated title :

Food Chem

Volume number :

373

Pages :

131690

Publication date :

2022-03-30

ISSN :

1873-7072

English keyword(s) :

Fish freshness
Flow cytometry
Mitochondria
Membrane potential
European seabass

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Mitochondria are real sensors of the physiological status of tissues. After the death of an animal, they maintain physiological activity for several days. This activity is highly dependent on the availability of nutrients ...
Show more >Mitochondria are real sensors of the physiological status of tissues. After the death of an animal, they maintain physiological activity for several days. This activity is highly dependent on the availability of nutrients in the tissue. In this study, flow cytometry was used to measure the membrane potential of mitochondria isolated from European seabass (Dicentrarchus labrax) red muscle stored in ice for seven days in order to characterize fish freshness. Two probes, TMRM and Rhodamine 123, were used to measure mitochondrial potential. During the first few days (D0 to D3), isolated mitochondria maintained high potential, and then lost their potential (from D3 to D5), but were always re-polarizable after addition of substrates (glutamate, malate and succinate). From D7, the mitochondria were more strongly depolarized and were difficult to repolarize by the substrates. Using flow cytometry, we demonstrated that mitochondria were an excellent marker to confirm seabass freshness.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41

Research team(s) :

Plateforme BioImaging Center Lille (BICeL)

Submission date :

2023-12-21T07:04:29Z
2024-02-23T11:37:58Z

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Version	Link	Modification date
2	20.500.12210/95745*	2024-02-23T11:26:15Z
1	20.500.12210/95745.1	2023-12-21T07:04:29Z

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Measurement of fish freshness: Flow cytometry ...

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