Measurement of fish freshness: Flow cytometry analysis of isolated muscle mitochondria.

Soret, Meline; Bouchendhomme, T.; Cleach, J.; Jouy, Nathalie; Crola Da Silva, C.; Devin, A.; Grard, Thierry; Lencel, P.

Type de document :

Article dans une revue scientifique: Article original

DOI :

10.1016/j.foodchem.2021.131690

PMID :

34865931

URL permanente :

http://hdl.handle.net/20.500.12210/95745

Titre :

Measurement of fish freshness: Flow cytometry analysis of isolated muscle mitochondria.

Auteur(s) :

Soret, Meline [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Bouchendhomme, T. [Auteur]
Cleach, J. [Auteur]
Jouy, Nathalie [Auteur]
Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41
Plateforme BioImaging Center Lille - PLBS [BICeL]
Crola Da Silva, C. [Auteur]
Devin, A. [Auteur]
Grard, Thierry [Auteur]

BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Lencel, P. [Auteur]

Titre de la revue :

Food Chemistry: X

Nom court de la revue :

Food Chem

Numéro :

373

Pagination :

131690

Date de publication :

2022-03-30

ISSN :

1873-7072

Mot(s)-clé(s) en anglais :

Fish freshness
Flow cytometry
Mitochondria
Membrane potential
European seabass

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Mitochondria are real sensors of the physiological status of tissues. After the death of an animal, they maintain physiological activity for several days. This activity is highly dependent on the availability of nutrients ...
Lire la suite >Mitochondria are real sensors of the physiological status of tissues. After the death of an animal, they maintain physiological activity for several days. This activity is highly dependent on the availability of nutrients in the tissue. In this study, flow cytometry was used to measure the membrane potential of mitochondria isolated from European seabass (Dicentrarchus labrax) red muscle stored in ice for seven days in order to characterize fish freshness. Two probes, TMRM and Rhodamine 123, were used to measure mitochondrial potential. During the first few days (D0 to D3), isolated mitochondria maintained high potential, and then lost their potential (from D3 to D5), but were always re-polarizable after addition of substrates (glutamate, malate and succinate). From D7, the mitochondria were more strongly depolarized and were difficult to repolarize by the substrates. Using flow cytometry, we demonstrated that mitochondria were an excellent marker to confirm seabass freshness.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41

Équipe(s) de recherche :

Plateforme BioImaging Center Lille (BICeL)

Date de dépôt :

2023-12-21T07:04:29Z
2024-02-23T11:37:58Z

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Numéro de version	Lien	Date de modification
2	20.500.12210/95745*	2024-02-23T11:26:15Z
1	20.500.12210/95745.1	2023-12-21T07:04:29Z

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Measurement of fish freshness: Flow cytometry ...

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